Time period | Control | Ifosfamide (µM)1 |
|---|
25 | 50 | 100 |
|---|
48 h | 3.1 ± 0.1a | 8.2a ± 0.9 | 13.2a ± 1.1 | 18.6a ± 1.5 |
72 h | 3.5 ± 0.9b | 10.4*,b ± 0.6 | 16.8*,b ± 1.3 | 24.5*,b ± 2.1 |
- Data represent mean ± SE, n = 3
- 1Ifosfamide was used in its IC50 value beside two of its fractions; 25 and 50 µM
- The number of HepG2 cells used was in the range of 5 × 105 ~ 5 × 106 cells/mL
- Percent apoptosis was calculated as the sum of frequencies of cells in late apoptosis and those undergoing apoptosis
- *Significantly different from the same concentration incubated with HepG2 cells for 48 h at P < 0.05, using independent Student’s t test
- a,bData in the same rows having the same superscript are significantly different from each other at P < 0.05 using one-way analysis of variance (ANOVA) followed by Tukey as post hoc test
