Time period | Control | Glufosfamide (µM)1 |
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10 | 25 | 50 |
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48 h | 2.4 ± 0.04 | 1.02a ± 0.01 | 0.9a ± 0.02 | 0.79a ± 0.01 |
72 h | | 0.9*,b ± 0.02 | 0.73*,b ± 0.05 | 0.57*,b ± 0.02 |
- Data represent mean ± SE, n = 4
- 1Glufosfamide was used in its IC50 value beside two of its fractions; 10 and 25 µM
- The number of HepG2 cells used was in the range of 5 × 105 ~ 5 × 106 cells/mL
- Bcl2 content was normalized to the reference β-actin gene and expressed as percent expression relative to untreated control cells
- *Significantly different from the same concentration incubated with HepG2 cells for 48 h at P < 0.05, using independent Student’s t test
- a,bData in the same rows having the same superscript are significantly different from each other at P < 0.05 using one-way analysis of variance (ANOVA) followed by Tukey as post hoc test