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Table 6 Effects of glufosfamide on Bcl2 gene expression in HepG2 cells

From: An in vitro cytotoxicity of glufosfamide in HepG2 cells relative to its nonconjugated counterpart

Time period Control Glufosfamide (µM)1
10 25 50
48 h 2.4 ± 0.04 1.02a ± 0.01 0.9a ± 0.02 0.79a ± 0.01
72 h   0.9*,b ± 0.02 0.73*,b ± 0.05 0.57*,b ± 0.02
  1. Data represent mean ± SE, n = 4
  2. 1Glufosfamide was used in its IC50 value beside two of its fractions; 10 and 25 µM
  3. The number of HepG2 cells used was in the range of 5 × 105 ~ 5 × 106 cells/mL
  4. Bcl2 content was normalized to the reference β-actin gene and expressed as percent expression relative to untreated control cells
  5. *Significantly different from the same concentration incubated with HepG2 cells for 48 h at P < 0.05, using independent Student’s t test
  6. a,bData in the same rows having the same superscript are significantly different from each other at P < 0.05 using one-way analysis of variance (ANOVA) followed by Tukey as post hoc test