Table 1 Primers, product size and annealing temperatures used to detect mutations, if any, in various exons of EGFR gene by ARMS-PCR and AS-PCR

Exon 19

15 bp deletion; codons 746–750

P-5′-GTAACATCCACCCAGATCACTG-3′

Q-5′-GTGTCAAGAAACTAGTGCTGGG-3′

A-5′-CCCGTCGCTATCAAGGAATTAA-3′

B-5′-GTTGGCTTTCGGAGATGTTTTGATAG-3′

60

(Single tube reaction)

PQ = 444 bp (control)

AQ = 325 bp (deletion absent)

PB = 134 bp (deletion present)

Exon 20

T790M

E-5′-GAAGCCACACTGACGTGCCT-3′

F-5′-GCCGAAGGGCATGAGCTGTG-3′

G-5′-ACCATGCGAAGCCACACTGACG-3′

H-5′-GCCGAAGGGCATGAGCTGGA-3′

56

(Two tube reaction)

EF = 139 bp (for wild allele)

GH = 146 bp (for variant allele)

Exon 21

L858R (T2573G)

P-5′-GGGTCTTCTCTGTTTCAGGGCAT-3′

A-5′-TTCCGCACCCAGCAGTTTGGCTA-3′

B-5′-CGCACCCAGCAGTTTGGTTC-3′

60

(Two tube reaction)

PA = 137 bp (wild allele)

PB = 134 bp (variant allele)