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Table 1 Immunohistochemistry conditions and evaluation

From: Significance of p53, p27, Ki-67, E-cadherin, and HER2 expression in upper urinary tract urothelial carcinoma

Expression Provenance Clone Dilution Retrieval solution Positive immunostaining
p53 DAKO Do-7 1:50 Citrate 0.01 M pH 6 Nuclear staining in ≥ 50% of tumor cells
p27 DAKO Sx5368 1:25 Citrate 0,01 M pH 9 Nuclear staining in ≥ 10% of tumor cells
Ki-67 DAKO Mib1 1:50 Citrate 0,01 M pH 6 Nuclear staining in ≥ 10% of tumor cells
E-cadherin DAKO NCH-38 1:30 Citrate 0,01 M pH 6 Membranous staining in ≥ 10% of tumor cells
HER2 DAKO Polyclonal (A0485) 1:300 Citrate 0,01 M pH 6 Membranous staining in ≥ 30% of tumor cells