Table 1 Immunohistochemistry conditions and evaluation
Expression | Provenance | Clone | Dilution | Retrieval solution | Positive immunostaining |
|---|---|---|---|---|---|
p53 | DAKO | Do-7 | 1:50 | Citrate 0.01 M pH 6 | Nuclear staining in ≥ 50% of tumor cells |
p27 | DAKO | Sx5368 | 1:25 | Citrate 0,01 M pH 9 | Nuclear staining in ≥ 10% of tumor cells |
Ki-67 | DAKO | Mib1 | 1:50 | Citrate 0,01 M pH 6 | Nuclear staining in ≥ 10% of tumor cells |
E-cadherin | DAKO | NCH-38 | 1:30 | Citrate 0,01 M pH 6 | Membranous staining in ≥ 10% of tumor cells |
HER2 | DAKO | Polyclonal (A0485) | 1:300 | Citrate 0,01 M pH 6 | Membranous staining in ≥ 30% of tumor cells |