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Table 4 Effects of glufosfamide on caspase-9 content in cell lysate of cultured HepG2 cells

From: An in vitro cytotoxicity of glufosfamide in HepG2 cells relative to its nonconjugated counterpart

Time period Control Glufosfamide (µM)1
10 25 50
48 h 4.0 ± 0.2a 15.1a ± 1.3 22.5a ± 1.2 54.5a ± 2.5
72 h 4.2 ± 0.5b 23.13*,b ± 1.9 35.0*,b ± 2.3 72.5*,b ± 4.1
  1. Data represent mean ± SE, n = 3
  2. 1Glufosfamide was used in its IC50 value beside two of its fractions; 10 and 25 µM
  3. The number of HepG2 cells used was in the range of 5 × 105 ~ 5 × 106 cells/mL
  4. Caspase-9 content was expressed as ng/mL
  5. *Significantly different from the same concentration incubated with HepG2 cells for 48 h at P < 0.05, using independent Student’s t test
  6. a,bData in the same rows having the same superscript are significantly different from each other at P < 0.05 using one way analysis of variance (ANOVA) followed by Tukey as post hoc test