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Table 7 Effects of ifosfamide on Bcl2 gene expression in HepG2 cells

From: An in vitro cytotoxicity of glufosfamide in HepG2 cells relative to its nonconjugated counterpart

Time period Control Ifosfamide (µM)1
25 50 100
48 h 2.4 ± 0.04 1.2a ± 0.01 0.99a ± 0.03 0.85a ± 0.02
72 h   1.03*,b ± 0.009 0.87*,b ± 0.02 0.75*,b ± 0.01
  1. Data represent mean ± SE, n = 3
  2. 1Ifosfamide was used in its IC50 value beside two of its fractions; 25 and 50 µM
  3. The number of HepG2 cells used was in the range of 5 × 105 ~ 5 × 106 cells/mL
  4. Bcl2 content was normalized to the reference β-actin gene and expressed as percent expression relative to untreated control cells
  5. *Significantly different from the same concentration incubated with HepG2 cells for 48 h at P < 0.05, using independent Student’s t test
  6. a,bData in the same rows having the same superscript are significantly different from each other at P < 0.05 using one-way analysis of variance (ANOVA) followed by Tukey as post hoc test