Time period | Control | Ifosfamide (µM)1 |
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25 | 50 | 100 |
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48 h | 2.4 ± 0.04 | 1.2a ± 0.01 | 0.99a ± 0.03 | 0.85a ± 0.02 |
72 h | | 1.03*,b ± 0.009 | 0.87*,b ± 0.02 | 0.75*,b ± 0.01 |
- Data represent mean ± SE, n = 3
- 1Ifosfamide was used in its IC50 value beside two of its fractions; 25 and 50 µM
- The number of HepG2 cells used was in the range of 5 × 105 ~ 5 × 106 cells/mL
- Bcl2 content was normalized to the reference β-actin gene and expressed as percent expression relative to untreated control cells
- *Significantly different from the same concentration incubated with HepG2 cells for 48 h at P < 0.05, using independent Student’s t test
- a,bData in the same rows having the same superscript are significantly different from each other at P < 0.05 using one-way analysis of variance (ANOVA) followed by Tukey as post hoc test