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Journal of the Egyptian National Cancer Institute
Fig. 4 | Journal of the Egyptian National Cancer Institute

Fig. 4

From: In vitro synergistic effect of hesperidin and doxorubicin downregulates epithelial-mesenchymal transition in highly metastatic breast cancer cells

Fig. 4

The activity of MMP-9 and the expression of Rac-1 after Hsd, DOX, and combination treatments on 4T1 breast cancer cells. The 4T1 cells were incubated with test samples for 24 h for further isolation and assays. A The MMP activity of MMP 9 and pro MMP 9 were analyzed using gelatin zymography. B The quantification of MMP 9 expression levels under gelatine zymography. C Rac-1 expression level of cell migration-related proteins was measured by western blotting. The quantification of band intensity was calculated with ImageJ software and presented as the graph ratio compared with untreated. The data were shown as the mean ± SD of a triplicate independent experiment. *p < 0.001 (post-hoc LSD test between each group) was considered statistically significant

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