Table 2 The RT-PCR reaction profile
Step | Time | Temperature (°C) | Cycles |
|---|---|---|---|
Initial denaturation | 30 seconds | 95 | 1 |
Denaturation | 5 seconds | 95 | 40 |
Annealing | 30 seconds | 55 | |
Extension | 45 seconds | 72 | |
Final extension | 5 minutes | 72 | 1 |
Step | Time | Temperature (°C) | Cycles |
|---|---|---|---|
Initial denaturation | 30 seconds | 95 | 1 |
Denaturation | 5 seconds | 95 | 40 |
Annealing | 30 seconds | 55 | |
Extension | 45 seconds | 72 | |
Final extension | 5 minutes | 72 | 1 |