Fig. 1
From: Significance of OCT3/4 and SOX2 antigens expression by leukemic blast cells in adult acute leukemia
Immunophenotyping of ALL case. A Time versus SS to ensure continuity of cell flow. B FS peak versus FS integral to include only singlet cells. C CD45 versus FS to exclude debris and dead cells. D CD45 versus SS to gate blast cells in low SS and dim CD45. E Positive CD19, CD10 expression on blast cells. F Positive CD58, CD81 expression on blast cells. G Positive CD34 expression on blast cells. H Negative CD117 expression on blast cells. I Negative CD13 expression on blast cells. J Aberrant CD33 expression on blast cells. K Positive CD79a expression on blast cells. L SOX2 versus SS to calculate the expression of SOX2 as a percent of blast cells in the A4 compartment. M OCT3/4 versus SS to calculate expression of OCT3/4 as percent of blast cells in B4 compartment